Oxidative stress plays an important role in the decline of sperm quality during chilled storage through the production of reactive oxygen species (ROS) such as hydrogen peroxide, H₂O₂. This study evaluated the effect of two antioxidants, methionine and quercetin, on rabbit sperm quality and H₂O₂ production during liquid storage over 96 h. Semen was collected from adult bucks (n=12) and pooled at 37°C following evaluation. In Experiment 1, pooled ejaculates were diluted with a Tris-based extender supplemented with methionine (1mM, 6mM or 12 mM), quercetin (50µM or 200µM) or no antioxidant (control) and then subdivided for storage at 5°C or 15°C. Sperm quality was assessed by CASA (total motility [TM]) and flow cytometry (viability, acrosome integrity and H₂O₂ production) at 0, 48, 72 and 96 h. Data were analysed using a Linear Mixed Model (P<0.05). During storage, TM was higher in control samples, and lowest following dilution with 200µM quercetin, irrespective of storage temperature (P<0.05).  Storage at 15°C improved viability and acrosome integrity (P<0.05) compared with 5°C, but resulted in significantly higher levels of intracellular H₂O₂ at 72 and 96 h in sperm diluted in methionine or the control extender. Spermatozoa supplemented with quercetin exhibited lower levels of H₂O₂ at both storage temperatures for all incubation times (P>0.05). In Experiment 2, the concentration of quercetin (0, 25, 50, 100 and 200 µM) was investigated in respect to TM, viability, acrosome integrity, H₂O₂ production, lipid peroxidation and DNA integrity. All concentrations of quercetin provided a protective effect, reducing H₂0₂ during storage at 15°C. Only supplementation with 100 and 200 µM quercetin resulted in similar levels of H₂0₂ at 5°C and 15°C after 96 h (P>0.05). Quercetin also significantly reduced lipid peroxidation during storage at 15°C. However, it was not beneficial for TM, viability, acrosome or DNA integrity. Overall, supplementation of quercetin to sperm medium provided protection against oxidative stress in 15°C-stored rabbit spermatozoa over 96 h.