Declines of amphibians in the last three decades have placed them at the forefront of global biodiversity loss.  34% of amphibian species are at risk of extinction.  The main driver, besides habitat loss, is the global pandemic of chytridiomycosis, often associated with the rapid decline and extinction of populations and species.  Global resources for captive breeding are limited, and capable of sustaining only a limited number of species in ex situ colonies, leaving biobanking as an essential , but not yet utilised, tool to prevent loss of genetic diversity and species.   The obvious target for cryopreservation of amphibians, particularly frogs, would be the early embryo, as fertilisation and development is external.  However, this is unlikely to succeed using currently available protocols.  The record of sperm cryopreservation is much better, and there are growing numbers of species that have been successfully cryopreserved, using recovery of motility, and even fertilisation post-thaw as the criteria. Our group at the University of Newcastle was the first to report fertilisation utilising cryopreserved amphibian sperm (in Rhinella marina, a bufonid from Central/South America).  Since then, other laboratories have reported similar outcomes in limited numbers of species.   The protocol we developed utilises high concentrations of osmoticum and cryoprotectant, and slow cooling during cryopreservation.  The question going forward is what use can be made of cryopreservation technology that allows sperm freezing, but not the cryopreservation of mature oocytes, eggs or embryos?  The answer, in the short to medium term, may lay in the use of androgenesis.  With androgenesis, doubled haploid embryos are generated by fertilisation of enucleated eggs with viable sperm, generating haploid zygotes subsequently transformed into viable diploid (doubled haploid) embryos by delay of first cleavage.  Our group is currently exploring the possible application of androgenesis with the goal of its application to biobanking endangered species.